Metrohm 850 Professional IC AnCat MCS Manuel d'utilisateur

850 Professional ICAnCat – MCS – 2.850.3030Manual8.850.8052EN

Table of figures￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VIII￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSFigure 46 MSM – Components ...

4.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS123 45Figure 46 MSM – Components1Union nut2MSM co

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭91￭ Check whether solution emerges at the MSM connecting pie

4.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS4.11.3.3 Replacing parts of the MSMIt may be nec

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭93￭ Insert the new MSM rotor A (46-3) in the MSM housing (46

4.12 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSCautionThe 6.1826.xxx pump tubings consist of PVC or PP and ther

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭95OrdernumberName Material InnerdiameterUse6.1826.330 Pump t

4.13 Metrohm CO2 suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS 3Mounting filter screw￭ Screw the filter screw (47-

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭974.14 Conductivity detector4.14.1 MaintenanceCautionThe c

4.15 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS4.15.2 ProtectionTo protect the separation column against fore

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭994.16 Quality Management and validation with MetrohmQualit

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe instrument 850 Professiona

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS5 Troubleshooting5.1 Problems and their solutionsP

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Troubleshooting850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭101Problem Cause RemedyEluent – Evaporation of theorganic solvent in t

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSProblem Cause RemedyMCS – vacuum pumpdefective.Conta

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Troubleshooting850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭103Problem Cause Remedy￭ Replace separation column (see "Connect-

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSProblem Cause RemedyEluent – Gas bubbles in theeluen

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭1056 Technical specifications6.1 Reference conditionsThe te

6.5 Housing￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS6.5 HousingDimensionsWidth 365 mmHeight 642 mmDepth 380 mmMaterial of ba

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭107Pressure rangePump 0…50.0 MPa (0…500 bar)Pump head 0…35.0

6.10 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS6.10 Column thermostatType Peltier technique thermostat for t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭1096.13 Metrohm CO2 suppressor (MCS)Material fluoropolymerRe

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS3162184956121310711151417Figure 1 Front view of the instrume

6.15 Mains connection￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSCell tempera-ture20…50 °C in increments of 5 °CTemperaturestabi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭1116.17 Safety specificationDesign / Test ￭ EN/IEC/UL 61010-

7.1 Declaration of Conformity￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS7 Conformity and warranty7.1 Declaration of Conformit

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Conformity and warranty850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭113EN 61010-1 Safety requirements for electrical equipment for

7.3 Warranty (guarantee)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭114￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSSoftware developmentSoftware development occurs in terms of

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Conformity and warranty850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭115Faults arising from circumstances that are not the responsi

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭116￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS8 AccessoriesCautionSubject to change without notice!8.1 Scop

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭117Qty. Order no. Description2 6.1608.020 Glass bottle / 1000 mL / GL 45Bo

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭118￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSQty. Order no. Description1 6.1803.040 PTFE capillary 0.5 mm i.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭119Qty. Order no. Description2 6.1816.020 Silicone tubing 6 mm i.d. / 1 mF

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭35MSMSee Chapter 2.16.6Injection valveSee Chapter 2.14.7Eluent degasserSe

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭120￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSQty. Order no. Description1 6.2151.020 Cable USB A - USB B / 1.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭121Qty. Order no. Description1 6.2621.030 Hexagon key 4 mm4 mm. For IC Sam

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭122￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSQty. Order no. Description1 6.2626.000 Front Drain NozzleDrain

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭123Qty. Order no. Description2 6.2744.020 Luer/UNF couplingFor IC instrume

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭124￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSQty. Order no. Description3 6.2744.070 Pressure screw shortShor

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭125Qty. Order no. Description2 6.2816.020 Syringe 10 mL with Luer connecti

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭126￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSQty. Order no. Description1 6.2837.000 CO2 adsorber cartridgeAd

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭1278.2 Optional accessories 2.850.3030 850 Professional IC– AnCat – MCSO

8.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭128￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSOrder no. Description6.1826.360 Pump tubing LFL (white/white

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭129Order no. Description6.2148.010 Remote box MSBAn additional remote inte

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSInjection valveThe injection valve connects the eluent and s

8.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭130￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSOrder no. Descriptiontrol; very flexible user administration

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭131IndexNumbers/Symbols6.2821.090 aspiration filter ... 726.2821.130 filter ...

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭132￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSValves ... 81Housing ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭133Safety specification ... 111SampleCarry-over ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭51.2 Intended useThe 850 Professional IC – AnCat – MCS is used for the i

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSWarningThis symbol draws attention to a possible hazard dueto h

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭7WarningNever open the housing of the instrument. The instrument could be

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS1.4.4 Flammable solvents and chemicalsWarningAll relevant safe

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭92 Installation2.1 About this chapterThe Installation chapter contains￭

2.2 Initial installation￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS 5Connect the leak sensor (see Chapter 2.6.4, page 21). 6Conn

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭112.3 Flow diagramFigure 2 Flow diagram for a 2-channel system shows the

2.3 Flow diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS718171920272316262824253245614113151112299303231331082122EluentSample

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭133High pressure pumpSee Chapter 2.10.4Purge valveSee Chapter 2.10.1.5Inl

2.4 Setting up the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS31Regeneration solution outputConnection to the waste co

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭15412 3Figure 3 Connection of capillaries with pressure screws 1PEEK pres

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSCautionFor the capillary connections between the injection

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭17321145Figure 4 Rollers and handle1Knurled screwsFor fastening the handl

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSMounting handle as MPak holderNoteWhen extended, the handle

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭192.6.2 Positioning and connecting the detectorDetector 1Detector 244443

Metrohm AGCH-9101 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional ICAnCat – MCS – 2.850.30

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSNoteUp to two detectors can be positioned and connected.Cau

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭21￭ Connect the detector cable to the detector connection socketDetector

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS123Figure 7 Connection for the leak sensor on the rear of t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭23245613798Figure 8 Drainage tubing1Drainage tubing connectionFor drainin

2.7 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS7Drainage tubingSection of the 6.1816.020 silico

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭25212134Figure 9 Capillary feed-throughs on the doors1Luer connectorsFor

2.7 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS1234567 88 76 59101112 1211109FrontRearFigure 10

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭272.8 Eluent2.8.1 Connecting eluent bottleThe eluent is aspirated out o

2.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS1 234Figure 11 Installing eluent bottle attachment1Eluent aspiration tubing

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭29 4Installing tubing weighting and aspiration filter1 2345Figure 13 Inst

TeachwareMetrohm AGCH-9101 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

2.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS￭ Fasten the bottle attachment (13-2) on the eluent bottle (15-10).The aspi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭315Eluent6Aspiration filter 6.2821.0907Filter holderFrom accessory set 6.

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS3Tubing flareWith tubing nipple.4Clamping screw5Eluent aspirat

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭3310891234562722211212213Figure 17 Capillary connections high pressure pu

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSNoteThe eluent aspiration tubing is already installed in the n

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭352.10.2 Deaerating the high pressure pumpThe high pressure pump will on

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSDeaerating the high pressure pumpThe instrument must be connec

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭37 4Setting the flow rate￭ Start MagIC Net (if not yet started).￭ Ensure

2.12 Pulsation damper￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSInstalling the inline filterCautionObserve the flow direction ma

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭39The pulsation damper protects the separation column from damagecaused b

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instrument description ...

2.13 Sample degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS2.13 Sample degasserThe sample degasser removes gas bubbles and

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭41 4Guide the other end of the connection capillary out of the instrument

2.14 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS3Connection capillaryConnected to connector 4. Carries eluent tot

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭43124 53 6124 53 6124354312A BFigure 24 Injection valve – PositionsAPosit

2.15 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS2.15 Column thermostatThe column thermostat controls the tempe

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭4513211112Figure 25 Column thermostat1Capillary feed-throughsFor guiding

2.15 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSNoteThe column input capillary is already threaded into the cap

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭472.16 Metrohm Suppressor Module (MSM)2.16.1 General information on the

2.16 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS13234567821Figure 26 MSM – connections1Union nut2

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭49Connecting the capillaries of the MSM 1Connecting the eluent input capi

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS2.14 Injection valve ...

2.17 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS2.17 Peristaltic pump2.17.1 Principle of the peristaltic pumpT

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭512.17.2 Installing the peristaltic pump6.2744.16016.2744.1802 3 4 5 6 7

2.17 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS 3Connecting the pressure sideNoteDepending on the use of the pe

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭53123Figure 30 Install pump tubing connection without filter1Union nut2Ad

2.17 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSPump tubing Adapter6.1826.340 (black/black)6.1826.360 (white/whi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭552.18 Metrohm CO2 suppressor (MCS)2.18.1 General information on the MC

2.18 Metrohm CO2 suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS3Aspirating capillaryFor aspirating air low in CO2 (

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭57938 7312 33456Figure 32 Adsorption cartridge holder1PEEK pressure screw

2.19 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS 2Removing the caps￭ Remove the two locking caps at the tip

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭59213Figure 33 Conductivity detector front1IC detector 1.850.90102Opening

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭V4.6 Inline filter ...

2.19 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS132Figure 34 Conductivity detector rear1Detector cableWith

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭613 21Figure 35 Connection detector – MCS1Detector input capillary2Pressu

2.21 Guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSThe mains cable is three-core and provided with a plug with groundin

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭63NoteThe guard column may only be installed after the initial start-up (

2.22 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS2.22 Separation columnThe intelligent separation column (iColu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭65Connecting and rinsing the separation column 1Connecting the separation

3.1 Initial start-up￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS3 Start-upThe chapter Start-up is divided into 2 sections:Initia

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Start-up850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭67 4Setting the contact pressure of the peristaltic pumpNoteThis work step ne

3.2 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS 2Preparing the instrument￭ Ensure that the column is correctly mount

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭694 Operation and maintenance4.1 General information4.1.1

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS6.10 Column thermostat ...

4.2 Capillary connections￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS4.1.3 OperationCautionIn order to avoid disturbing temperat

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭714.3 DoorCautionThe door is made of PMMA (polymethylmetacr

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS4.4.2 Operation4.4.2.1 Supply bottleThe supply bottle with th

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭73CautionIn order to spare the pump seals, the pump should n

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSCleaning/replacing the zirconium oxide pistonClean one piston a

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭75￭ Remove the backup ring from the pump head and lay to the

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSparts: a tip for removing the old piston seal and a sleeve for

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭7712Figure 39 Removing the piston seal1Piston seal2Tool for

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS12Figure 41 Inserting the piston seal into the pump head 4Repla

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭791234Figure 42 Removing valves1Outlet valve holder2Outlet v

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of figures850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭VIITable of figuresFigure 1 Front view of the instrument ...

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS￭ Place the valve with the seal faced downwards above the reces

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭81123567891098710546Figure 44 Components of the inlet valve

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS￭ Place the valve housing over the stacked components and hold

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭83Mounting the pump headNoteTo prevent the pump head from be

4.6 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSChanging the filterThe flow must be stopped before changing the filt

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭854.7 Inline sample preparationTo protect the separation co

4.8 Rinsing the sample path￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCS 2Aspirating the sample and measuring timeAspirate a sampl

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭874.9 Sample degasser4.9.1 OperationIf you are working wit

4.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭850 Professional IC – AnCat – MCSIn order to record every new chromatogram under c

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – AnCat – MCS￭￭￭￭￭￭￭￭89NoteThe high pressure pump can be used for regeneration. F