Metrohm 761 Compact IC Manuel d'utilisateur

761 Compact IC METROHM Ltd. CH-9101 Herisau Switzerland Phone ++41 71 353 85 85 Fax ++41 71 353 89 01 8.761.1063Instructions for Use

Table of contents 761 Compact IC VIII 79 4 Steel meshes ... 32 80 Manufit pressure screw ...

4 Operation 761 Compact IC 90 Name (1st column) User-definable name of the remote command (e.g. Start_766). Remote command (2nd column) Settin

4.3 Systems 761 Compact IC 91 Suppressor Suppressor module: Autostep with Automatic switching to the next position each time the injection valve

4 Operation 761 Compact IC 92 Flow correction Factor for correction of the difference between displayed and actual flow rate of the high-pressu

4.3 Systems 761 Compact IC 93 Event Events for automatic remote signal output: Inject Switching of the injection valve to the "INJECT"

4 Operation 761 Compact IC 94 Leak Leak detector has detected solvent in the in-struments interior. This information is also stored in the inst

4.3 Systems 761 Compact IC 95 Open Open the selected system. Close Close the selected system and disconnect it from the workplace. Startup Star

4 Operation 761 Compact IC 96 Error messages Detection of hard- ware failed Bad connection between PC and 761 Compact IC or instrument switched

4.4 Methods 761 Compact IC 97 4.4 Methods A method contains all information necessary for data acquisition, in-tegration, peak evaluation and quan

4 Operation 761 Compact IC 98 General Tab General of the passport with general description of the method and determination. Ident User define

4.4 Methods 761 Compact IC 99 To reset the Run number press [Ctrl] [F8]. The Analysis number window is opened where the run number for the next r

1.1 Instrument description 761 Compact IC 1 1 Introduction 1.1 Instrument description The 761 Compact IC is a PC-controlled system for ion chroma

4 Operation 761 Compact IC 100 Column Tab Column of passport with column information. Number Serial number of column (max. 256 characters). I

4.4 Methods 761 Compact IC 101 Eluent Tab Eluent of passport with eluent information. Eluent A Description of eluent composition (max. 256 char

4 Operation 761 Compact IC 102 Method Log Tab Method Log of passport with GLP Log for the method. The Method Log displays all automatically pro

4.4 Methods 761 Compact IC 103 Data Log Tab Data Log of passport with GLP Log for the recorded chromatogram. Here a GLP message is automatically p

4 Operation 761 Compact IC 104 4.4.3 Method setup 761 COMPACT IC / Method / Method setup This menu item opens the Method setup window which in

4.4 Methods 761 Compact IC 105 Filters Tab Filters of Method setup window with parameters for noise filtration of raw data. Three different filtra

4 Operation 761 Compact IC 106 Processing Tab Processing of Method setup window for definition of actions that are performed during the run or w

4.4 Methods 761 Compact IC 107 Math Tab Math of Method setup window with parameters that are used for vari-ous types of calculations. Parameter S

4 Operation 761 Compact IC 108 tion time replaces the previous value of the void time. If the first component is not identified, the expected re

4.4 Methods 761 Compact IC 109 4.4.4 Integration 761 COMPACT IC / Method / Integration This menu item opens the Integration parameters window wh

1 Introduction 761 Compact IC 2 The operation of the 761 Compact IC takes place via a PC connected to the RS232 interface with the help of the co

4 Operation 761 Compact IC 110 Setup Tab Setup of Integration parameters window with integration parameters. The two most important of these par

4.4 Methods 761 Compact IC 111 Broadening Broadening for peaks in the end of the chroma-togram compared to peaks in the beginning. This parameter

4 Operation 761 Compact IC 112 <Suggest> This function sets up reasonable integration pa-rameters in the following way (assumes that seve

4.4 Methods 761 Compact IC 113 If an integration event is added or modified, the Edit integration event window is opened where the following param

4 Operation 761 Compact IC 114 Stop single peak mode Disable peak end detection. All peaks of any group after this event will be treated as one

4.4 Methods 761 Compact IC 115 Set baseline point without Set baseline point at 4.5, 6, 7.2, 8.9 and 9.8 min Set baseline point Set d

4 Operation 761 Compact IC 116 Force horizontal base back Set horizontal baseline for a single peak. The baseline is drawn backwards from the pe

4.4 Methods 761 Compact IC 117 4.4.5 Calibration and quantification General information The aim of any chromatographic analysis is to answer the

4 Operation 761 Compact IC 118 Notations R Stands for response value, either area or height, de-pending on setting selected in the Calibration

4.4 Methods 761 Compact IC 119 External standard calibration External standard calibration (absolute calibration) is a key way of cali-bration. Ba

1.2 Parts and controls 761 Compact IC 3 1.2 Parts and controls POWER761 Compact IC89 76 5234110 Fig. 1: Front of the 761 Compact IC 1 Door to in

4 Operation 761 Compact IC 120 Number Row number (read-only). Peak Number of the peak in the chromatogram that cor-responds to the given com

4.4 Methods 761 Compact IC 121 Additionally, the Components table contains the following buttons: <Add> Add a new component (an empty colum

4 Operation 761 Compact IC 122 Identification Identification parameters. Reference peaks Identification parameter for reference compo-nents. D

4.4 Methods 761 Compact IC 123 Concentration table 761 COMPACT IC / Method / Calibration / Concentrations Selecting this menu item or clicking on

4 Operation 761 Compact IC 124 <Add> Add a new Calibration level to the concentration table. The following window appears: Create calibr

4.4 Methods 761 Compact IC 125 Calibration curve 761 COMPACT IC / Method / Calibration / Graphs Selecting this menu item or clicking on <Graph

4 Operation 761 Compact IC 126 Calibration points table This table contains the basic information used to construct the calibra-tion curve: Leve

4.4 Methods 761 Compact IC 127 Base Base for calculations (Area or Height) in quantifi-cation and calibration procedures. Indicates, which param

4 Operation 761 Compact IC 128 sponse factor of this component so, that the to-tal amount of the injected components be-comes equal to the decla

4.4 Methods 761 Compact IC 129 4.4.6 Report output Report options 761 COMPACT IC / Method / Report options 761 COMPACT IC / Process / Make rep

1 Introduction 761 Compact IC 4 WARNING - Fire Hazard -with the same type and rating of fuseFor continued protection replace onlyType 1.761.Fuse1

4 Operation 761 Compact IC 130 Sample Sample information from the Sample tab of the passport. SAMPLE: Tap water from Application Laborato

4.4 Methods 761 Compact IC 131 Comment User defined comment from the Comment tab of the passport. Method example for water determination. GLP L

4 Operation 761 Compact IC 132 Calibration defaults Calibration parameters defined in the compo-nent window and on the Math tab of the Method s

4.4 Methods 761 Compact IC 133 Channel table Parameters of the data acquisition channel used. CHANNELS TABLE Cond No Name

4 Operation 761 Compact IC 134 Peak table The following parameters can be selected for peak table output with de-termination results: Quantifica

4.4 Methods 761 Compact IC 135 and the concentration of this internal standard must be entered in the Concentration of std field. As long as no un

4 Operation 761 Compact IC 136 By components Missing components are always included into the report, even those with concentration equal to zer

4.4 Methods 761 Compact IC 137 Mode The two options Overwrite (overwrite the file) or Append (append to existing file) are available. Character s

4 Operation 761 Compact IC 138 R = (ti+1 - ti) / (w0.607i + w0.607(i+1)) where i and i+1 indexes refer to the neighboring peaks, and w0.607 stan

4.4 Methods 761 Compact IC 139 raw concentration Absolute concentrations (raw quantity) of the components, calculated as Ci = Wi(Ri) / V' T

1.2 Parts and controls 761 Compact IC 5 10 Bottle rack for holding supply bottles with eluent, regeneration solution, and rinsing solution 18 Tr

4 Operation 761 Compact IC 140 ? Some points of corresponding peak are out of ADC or detector range – suspicious result. ! Component concen

4.5 Chromatograms 761 Compact IC 141 4.5 Chromatograms 4.5.1 Chromatogram window The CHROMATOGRAM window is used to show a running or recorded ch

4 Operation 761 Compact IC 142 4.5.2 Chromatogram file handling Open chromatogram 761 COMPACT IC / File / Open / Chromatogram Load an existi

4.5 Chromatograms 761 Compact IC 143 Directories Box with the list of directories. Allows to change the working directory defined in the method,

4 Operation 761 Compact IC 144 Represent raw data as Output of raw data: Default units (float) Y values are exported in default units for the

4.5 Chromatograms 761 Compact IC 145 4.5.3 Graphical representation Appearance 761 COMPACT IC / View / Appearance This menu item opens the Appea

4 Operation 761 Compact IC 146 Response axis Y from Beginning of the window on Y axis. Y to End of the window on Y axis. Tic marks Plot axe

4.5 Chromatograms 761 Compact IC 147 Component name + Quantity Name and quantity of the component. Channel labels Label for data acquisition chan

4 Operation 761 Compact IC 148 Colors Tab Colors of the Appearance window with color settings parameters for chromatograms. this color is defau

4.5 Chromatograms 761 Compact IC 149 Other graphical functions 761 COMPACT IC / View / X full scale By selecting this menu item or pressing [ Ct

1 Introduction 761 Compact IC 6 37441262727282930313243424140393836 35 344334546 Fig. 3: Interior of the 2.761.0010 Compact IC (with permanently

4 Operation 761 Compact IC 150 4.5.4 Peak editor Switching on/off the peak editor 761 COMPACT IC / Process / Peak editor The peak editor mode

4.5 Chromatograms 761 Compact IC 151 761 COMPACT IC / Peak / Select end point Move the cursor to the end of the nearest peak and select the peak

4 Operation 761 Compact IC 152 4.5.5 Printing Page layout for printing 761 COMPACT IC / File / Page layout The selection of this menu item or cl

4.5 Chromatograms 761 Compact IC 153 Printer settings 761 COMPACT IC / File / Printer setup By selecting this menu item the PRINT SETUP window is

4 Operation 761 Compact IC 154 Subtraction of a chromatogram 761 COMPACT IC / Process / More / Subtract This menu item opens the Subtract chro

4.5 Chromatograms 761 Compact IC 155 Autodatabase options The «Autodatabase 1.0» PC program is a part of the 761 Compact IC standard accessories

4 Operation 761 Compact IC 156 4.6 Sample queue A sample queue is a table containing sample-specific data which is used to facilitate work with

4.6 Sample queue 761 Compact IC 157 4.6.2 Sample queue control Sample queue overview table 761 COMPACT IC / File / Open / Sample queue SYSTEM

4 Operation 761 Compact IC 158 Started Indication whether the sample has been started or not: 0 sample not started 1 sample started 2…n n

4.6 Sample queue 761 Compact IC 159 Pause sample queue SAMPLE QUEUE OVERVIEW / Control / Pause Interrupt execution of the sample queue after the

1.2 Parts and controls 761 Compact IC 7 1 Door to interior 36 Filter unit PEEK (6.2821.120) 4 Aspirating tubing for aspirating the sample; 6.18

4 Operation 761 Compact IC 160 No Row number. System System file to be used for the determination. The system is automatically opened and con-n

4.6 Sample queue 761 Compact IC 161 Sample queue editor functions The functions in the editor window for sample queues can be triggered with the c

4 Operation 761 Compact IC 162 4.7 Batch reprocessing Reprocessing is understood to be the subsequent reprocessing of a series of chromatograms

4.7 Batch reprocessing 761 Compact IC 163 4.7.2 Perform batch reprocessing Reprocess options window 761 COMPACT IC / File / Open / Batch reproc

4 Operation 761 Compact IC 164 <Edit sample table> Open the batch reprocessing editor program for editing the batch reprocessing table (s

4.7 Batch reprocessing 761 Compact IC 165 Recalculate only Enable recalculation of chromatograms with values for Volume, Dilution, Amount and Int

4 Operation 761 Compact IC 166 <Reprocess> Start reprocessing. <Merge> Combine all chromatograms of the batch re-processing queue i

4.7 Batch reprocessing 761 Compact IC 167 4.7.3 Batch reprocessing queue editor Open batch reprocessing queue editor window REPROCESS / <Edit

4 Operation 761 Compact IC 168 of this column enables to calculate calibration coefficients automatically. Sample Info 1 First sample descript

5.1 Practical notes on ion chromatography 761 Compact IC 169 5 Notes – Maintenance – Faults 5.1 Practical notes on ion chromatography 5.1.1 Separ

1 Introduction 761 Compact IC 8 37441262727282930313243424140393836 35 34433454647484953 525051 Fig. 4: Interior of the 2.761.0020 Compact IC (w

5 Notes – Maintenance – Faults 761 Compact IC 170 Storage Always store the separating columns closed when not in use and filled in accordance wit

5.1 Practical notes on ion chromatography 761 Compact IC 171 5.1.3 Eluents Treatment For the preparation of the eluents only chemicals of a purity

5 Notes – Maintenance – Faults 761 Compact IC 172 5.1.5 Suppressor module Protection To avoid contamination of the suppressor module by foreign p

5.2 Maintenance and servicing 761 Compact IC 173 5.2 Maintenance and servicing 5.2.1 General information Care The 761 Compact IC requires proper c

5 Notes – Maintenance – Faults 761 Compact IC 174 5.2.3 Recycling To keep the eluent consumption between injections to a minimum when the system

5.2 Maintenance and servicing 761 Compact IC 175 2 Connect new column to injector • Remove end caps from column 81. • Screw inlet end of separ

5 Notes – Maintenance – Faults 761 Compact IC 176 5.2.6 Maintenance work at the pump head In many cases, an unstable baseline (pulsation, flow f

5.2 Maintenance and servicing 761 Compact IC 177 42104 105 106 107 108 109 110 111 113115114115112 Fig. 19: Components of the pump head 42116A1161

5 Notes – Maintenance – Faults 761 Compact IC 178 • To install a new piston seal 112 the special tool 117 is used. • First the new seal is i

5.2 Maintenance and servicing 761 Compact IC 179 6 Mounting the pump head • Reassemble the components of the pump head 42 as shown in Fig. 19. Ti

1.2 Parts and controls 761 Compact IC 9 1 Door to interior 39 Purge valve 4 Aspirating tubing for aspirating the sample; 6.1803.020 PTFE tubin

5 Notes – Maintenance – Faults 761 Compact IC 180 5.2.7 Regeneration of the suppressor module Regenerating a suppressor operating at reduced ca

5.2 Maintenance and servicing 761 Compact IC 181 • Set the flow at the high-pressure pump to 0.5 mL/min and rinse the suppressor unit with 1 mol/L

5 Notes – Maintenance – Faults 761 Compact IC 182 2 Dismantle suppressor • Unscrew nut 125 from suppressor holder 128. • Pull out connection

5.2 Maintenance and servicing 761 Compact IC 183 128127126125 Fig. 22: Assembling the suppressor 125 Screw nut 127 Suppressor rotor (6.2832.000

5 Notes – Maintenance – Faults 761 Compact IC 184 3 Clean suppressor rotor • Clean the sealing surface of new suppressor rotor 127 (6.2832.00

5.2 Maintenance and servicing 761 Compact IC 185 5.2.10 Exchanging the pump tubing The pump tubings used by the peristaltic pump are consumable ma

5 Notes – Maintenance – Faults 761 Compact IC 186 5.3 Faults and malfunctions 5.3.1 Error messages If any type of malfunction occurs during oper

5.3 Faults and malfunctions 761 Compact IC 187 Malfunction Cause Rectification Chromatograms with poor resolu-tion, change in the retention times

5 Notes – Maintenance – Faults 761 Compact IC 188 5.4 Diagnostic tests / Validation / GLP The requirements of GLP (Good Laboratory Practice) i

6.1 Technical data 761 Compact IC 189 6 Appendix 6.1 Technical data 6.1.1 Conductivity measurement Measurement range 1 0…1000 µS/cm (resolution:

CH-9101 Herisau/Switzerland Phone ++41 71 353 85 85 Fax ++41 71 353 89 01 Internet www.metrohm.ch E-Mail [email protected] 761 Compact IC Prog

1 Introduction 761 Compact IC 10 1.3 Information on the Instructions for Use Please read through these Instructions for Use carefully before yo

6 Appendix 761 Compact IC 190 6.1.3 Injection valve Actuator switching duration 100…150 ms Pressure resistance 25 MPa (250 bar) 6.1.4 High-

6.1 Technical data 761 Compact IC 191 6.1.5 Peristaltic pump Type 2-channel peristaltic pump Pump capacity Rotational speed 20 U/min at 50 Hz 24

6 Appendix 761 Compact IC 192 6.1.9 Remote interface Connector Dsub 25 pin (female) Circuit diagram for output lines 1…8 Assignment of outpu

6.1 Technical data 761 Compact IC 193 Safety directions The Instructions for Use include information and warnings which must be heeded by the use

6 Appendix 761 Compact IC 194 6.2 Standard equipment Subject to changes ! All dimensions are given in mm. The 761 Compact IC is available in tw

6.2 Standard equipment 761 Compact IC 195 Quant. Order No. Description 2.761.0010 2.761.0020 1 1 6.1609.000 CO2 Absorber tube incl. 6.2701.02

6 Appendix 761 Compact IC 196 Quant. Order No. Description 2.761.0010 2.761.0020 1 1 6.2122.0X0 Mains cable to customer's specificati

6.2 Standard equipment 761 Compact IC 197 Quant. Order No. Description 2.761.0010 2.761.0020 1 1 6.2744.020 Coupling 1⁄16" – Luer Coupli

6 Appendix 761 Compact IC 198 6.3 Optional accessories 6.3.1 Accessories for capillary connections Order No. Description 6.1825.XXX Sample

6.3 Optional accessories 761 Compact IC 199 6.3.2 High-pressure pump Order No. Description 6.2824.100 Pump head (metal-free) Complete, with fi

1.3 Information on the Instructions for Use 761 Compact IC 11 1.3.2 Notation and pictograms The following notations and pictograms (symbols) are u

6 Appendix 761 Compact IC 200 Order No. Description 6.2824.080 Outlet valve (metal-free) Spare part for 6.2824.100 Pump head 6.2824.090 Inle

6.3 Optional accessories 761 Compact IC 201 Order No. Description 6.1005.050 IC precolumn cartridge PRP-1 To prolong the service life of the

6 Appendix 761 Compact IC 202 Order No. Description 6.1007.000 IC cation column Nucleosil 5SA For the determination of divalent cations with

6.3 Optional accessories 761 Compact IC 203 Order No. Description 6.1012.X20 Sample pretreatment cartridge IC-Ag Cation exchanger in Ag+ form.

6 Appendix 761 Compact IC 204 Order No. Description 6.2828.000 Glass cartridge holder To hold the 6.1006.0020 Column cartridge METROSEP Anio

6.3 Optional accessories 761 Compact IC 205 6.3.4 Additional devices and cables Order No. Description 2.750.0010 750 Autosampler Sampler for the

6 Appendix 761 Compact IC 206 6.4 Warranty and conformity 6.4.1 Warranty The warranty on our products is limited to defects that are traceable

6.4 Warranty and conformity 761 Compact IC 207 6.4.2 EU Declaration of conformity EU Declaration of Conformity The METROHM AG company, Herisau,

6 Appendix 761 Compact IC 208 6.4.3 Certificate of conformity and system validation Certificate of Conformity and System Validation This is to

6.5 Index 761 Compact IC 209 6.5 Index1 1st component ... 107 1st peak...

1 Introduction 761 Compact IC 12 1.4 Safety notes 1.4.1 Electrical safety While electrical safety in the handling of the 761 Compact IC is assure

6 Appendix 761 Compact IC 210 Output ...128 Plot area ... 152 Pr

6.5 Index 761 Compact IC 211 Connection capillary 35 Connection of pulsation dampener 60... 25 Figure...

6 Appendix 761 Compact IC 212 Eluents Notes...171 EMC ...

6.5 Index 761 Compact IC 213 IC precolumn cartridge Nucleosil 5SA ... 202 IC precolumn cartridge PRP-1 ... 2

6 Appendix 761 Compact IC 214 Merge peaks ...151 Method ...98,142,167 Defin

6.5 Index 761 Compact IC 215 Power consumption ... 192 Power failure ... 94 Power on

6 Appendix 761 Compact IC 216 Replacement of piston seals...170 Replacing the suppressor ...183 Repo

6.5 Index 761 Compact IC 217 Set min height ... 116 Set normal baseline ... 114 Set peak

6 Appendix 761 Compact IC 218 Tic marks ...146 Time...81,98,99,113,120 Time

2.1 Overview 761 Compact IC 13 2 Installation 2.1 Overview 2.1.1 Flow chart The following flow chart provides an overview of all installation work.

2 Installation 761 Compact IC 14 63404244 4328 81 45 4636 353739666768386026 29 32304 Fig. 5: Connecting diagram for 2.761.0010 Compact IC witho

2.2 Setting up the instrument 761 Compact IC 15 2.2 Setting up the instrument 2.2.1 Packaging The 761 Compact IC is supplied together with the sep

2 Installation 761 Compact IC 16 • Insert the cable permanently attached to the detector block 46 in one of the openings 11 and the outlet capil

2.3 Attaching the accessories 761 Compact IC 17 2.3.4 Connection of the drain tube for bottle rack The 761 Compact IC has a connection at the rear

2 Installation 761 Compact IC 18 2.3.6 Filter unit PEEK One 6.2821.120 Filter unit PEEK (see Fig. 8) is already installed be-tween the high-pres

2.4 Mains connection 761 Compact IC 19 2.4 Mains connection Follow the instructions below for connecting to the power supply. If the instrument i

Table of contents 761 Compact IC I Table of contents 1 Introduction...

2 Installation 761 Compact IC 20 19220 - 240 V 100 - 120 V 2021220 - 240 V 100 - 120 V Fig. 9: Setting the mains voltage 2.4.2 Fuses One of the

2.5 Connection to the PC 761 Compact IC 21 2.5 Connection to the PC 2.5.1 Connecting cable Always switch off 761 Compact IC and PC before you con

2 Installation 761 Compact IC 22 The installed files (incl. system and method files) are generally not write-protected. To prevent these files f

2.5 Connection to the PC 761 Compact IC 23 • Click on Options / 761 Compact IC:COM1 to open the Links window: • Click on COM1 using the right

2 Installation 761 Compact IC 24 • Select the Connect to workplace menu item of the Control menu in this window. • If the connection between PC

2.6 High-pressure pump 761 Compact IC 25 2.6 High-pressure pump In order to avoid damage to the pump it must never be operated dry. Each time that

2 Installation 761 Compact IC 26 373936 35 6232266061 Fig. 10: Connection of the pulsation dampener 26 Inlet capillary for injector 6.1831.010

2.6 High-pressure pump 761 Compact IC 27 2.6.3 Connecting the eluent bottle The eluent supply line from the storage bottle to the high-pressure pu

2 Installation 761 Compact IC 28 3 Mount CO2 absorber tube • First place a piece of cotton wool 70 followed by CO2 ab-sorber 69 (e.g. Merck so

2.6 High-pressure pump 761 Compact IC 29 2.6.4 Deaerating the pump and rinsing the pulsation dampener The first time that it is started up the hig

Table of contents 761 Compact IC II 2.8 Suppressor module ...

2 Installation 761 Compact IC 30 4 Deaerate pump • Make sure that the aspirating tubing 63 for the high-pressure pump has been immersed in

2.7 Precolumns and separating columns 761 Compact IC 31 2.7 Precolumns and separating columns 2.7.1 General information on precolumns The use of

2 Installation 761 Compact IC 32 7473 75 76 77 76 75 78 755428 79 80 81 Fig. 12: Installation of precolumn cartridges with cartridge head 28 Co

2.7 Precolumns and separating columns 761 Compact IC 33 2.7.3 Precolumn glass cartridges with cartridge holder The precolumn glass cartridge METRO

2 Installation 761 Compact IC 34 8584 83 86 875428 8382 82 54 56 Fig. 13: Installation of precolumn glass cartridges with cartridge holder 28 C

2.7 Precolumns and separating columns 761 Compact IC 35 2.7.5 General information on separating columns New IC separating columns are normally f

2 Installation 761 Compact IC 36 2.7.7 Installation of the separating column without suppressor With the 2.761.0010 Compact IC without suppresso

2.7 Precolumns and separating columns 761 Compact IC 37 882727454632818828 Fig. 14: Installation of column without suppressor 2.7.8 Installation

2 Installation 761 Compact IC 38 2.8 Suppressor module 2.8.1 General information on suppressor module The Metrohm Suppressor Module MSM for che

2.8 Suppressor module 761 Compact IC 39 • Press contact pressure lever 49 on both tubing cartridges down as far as it will go. • Insert the pump

Table of contents 761 Compact IC III Menu options for PC icon... 83 Select processing meth

2 Installation 761 Compact IC 40 47951015091 5293923228464533499195363691911009089974898995153279688812788 Fig. 16: Connection of the separating

2.8 Suppressor module 761 Compact IC 41 47 Suppressor module 95 PTFE tubing (6.1803.020) 48 Tubing cartridge (6.2755.000) for pump tubings 92/9

2 Installation 761 Compact IC 42 pump tubing 92. • Mount a 6.2744.010 Compression fitting at the end of the aspirating tubing 90 and screw this

2.8 Suppressor module 761 Compact IC 43 2.8.4 Connection of the suppressor module The three inlets and outlets numbered 1...3 on the suppressor mo

2 Installation 761 Compact IC 44 5 Inlet capillary for H2O • Attach inlet capillary 98 marked with "H2O" of suppressor module 47 us

2.9 Putting into operation 761 Compact IC 45 2.9 Putting into operation 2.9.1 Putting into operation without suppressor Before sample solutions c

2 Installation 761 Compact IC 46 3 Start system • Make sure, that the aspirating tubing 63 for the high-pressure pump is immersed in the eluen

2.9 Putting into operation 761 Compact IC 47 parameters. 3 Start system • Make sure, that the aspirating tubing 63 for the high-pressure pump

2 Installation 761 Compact IC 48 6 Condition system • Rinse the system with eluent until the desired stability of the baseline is reached (nor

2.10 Connection of external devices 761 Compact IC 49 2.10 Connection of external devices 2.10.1 Connection of the 750 Autosampler The 750 Autosa

Table of contents 761 Compact IC IV Appearance... 145 Other graphical

2 Installation 761 Compact IC 50 4 Settings at the 750 Autosampler • Set parameter "Run time" to 0.0 to activate the external start

2.10 Connection of external devices 761 Compact IC 51 • Connect the end of the 6.2141.110 Cable marked with "732/1" to the remote interf

2 Installation 761 Compact IC 52 5 Settings in the «761 Compact IC» program • A time program must be drawn up for the selected system which f

3.1 Requirements 761 Compact IC 53 3 Operating tutorial This section introduces you to the operation of the 761 Compact IC by means of a brief

3 Operating tutorial 761 Compact IC 54 3.2 Preparations Before you start this brief tutorial, the entire IC system must be correctly installed a

3.3 Calibration 761 Compact IC 55 3.3 Calibration After the complete IC system has been installed as described in sec-tion 3.2, the first calibrat

3 Operating tutorial 761 Compact IC 56 module is switched to the next position every 20 min and conditioned in this way. 5 Condition system &

3.3 Calibration 761 Compact IC 57 9 Enter information for determination ⇒ Click on or select Passport of the Method menu. The passport op

3 Operating tutorial 761 Compact IC 58 11 Fill sample loop ⇒ Immerse the aspirating tubing 4 attached to connection 3 in the standard solutio

3.3 Calibration 761 Compact IC 59 ⇒ Click on <Apply>. The Integration parameters window is opened and the chromatogram is reintegrated. ⇒

Table of contents 761 Compact IC V 5 Notes – Maintenance – Faults...169 5.1 Practical notes on io

3 Operating tutorial 761 Compact IC 60 This is done by clicking on the chromatogram and moving the cursor with the aid of the cursor keys <

3.3 Calibration 761 Compact IC 61 ⇒ Confirm Calibration level 1 in this window with <OK>. A new column Level 1 is added in the Concentrati

3 Operating tutorial 761 Compact IC 62 17 Display calibration curves ⇒ Click on <Graphs> in the Components window. ⇒ Select the desire

3.4 Sample determination 761 Compact IC 63 3.4 Sample determination Following the calibration of the IC system as described in section 3.3 the fir

3 Operating tutorial 761 Compact IC 64 5 Fill sample loop ⇒ Immerse the aspirating tubing 4 attached to connection 3 in the vessel containin

3.4 Sample determination 761 Compact IC 65 7 Open chromatogram ⇒ Click on or File / Open / Chromatogram in the main win-dow. Select the chr

3 Operating tutorial 761 Compact IC 66 9 Print report ⇒ Click on or select Print of the File menu in the main window. The standard printi

4.1 Fundamentals of the operation 761 Compact IC 67 4 Operation This section describes the most important points concerning the operation of the

4 Operation 761 Compact IC 68 4.1.2 Glossary System The term “system“ is used to describe the combination of instrument settings, time program a

4.1 Fundamentals of the operation 761 Compact IC 69 Integration Integration is to be understood as being the method for determining the peak area

Table of contents 761 Compact IC VI List of figures Fig. 1: Front of the 761 Compact IC ...

4 Operation 761 Compact IC 70 4.1.4 Main window elements The elements of the main window 761 COMPACT IC are the menu bar, the tool bar and the

4.1 Fundamentals of the operation 761 Compact IC 71 Enable/disable peak editor mode View whole chromatogram Help Lock system Connected syst

4 Operation 761 Compact IC 72 4.1.7 Context sensitive menus Some of the menu functions of the program windows are also accessi-ble by clicking

4.1 Fundamentals of the operation 761 Compact IC 73 [ Ctrl ] + [ right ] Moves one window right (without change of scale on X and Y axes). [ Ctr

4 Operation 761 Compact IC 74 4.2 Instrument and software settings 4.2.1 Fonts 761 COMPACT IC / Options / Fonts This option allows the selectio

4.2 Instrument and software settings 761 Compact IC 75 Enter user name, password and access level for all users. Don't forget to make one o

4 Operation 761 Compact IC 76 Data file overwrite = Never If method changed = Don't ask to save If disk method is newer = Ask to o

4.2 Instrument and software settings 761 Compact IC 77 Chromatogram units Units for chromatograms: Flow Unit for flow rate: µL/min, mL/min Press

4 Operation 761 Compact IC 78 4.3 Systems The term “System“ describes the combination of instrument settings, time program and process method wh

4.3 Systems 761 Compact IC 79 SYSTEM / System / Save Save the current system settings in a system file (*.smt) in the Systems directory. SYSTEM /

Table of contents 761 Compact IC VII List of numbered parts and controls 1 Door to interior ...3,7,9 2 Connection

4 Operation 761 Compact IC 80 SYSTEM / Control / Shutdown hardware High-pressure pump and (if present) peristaltic pump at the 761 Com-pact IC a

4.3 Systems 761 Compact IC 81 Ident User defined identifier (title) for the chroma-togram to be displayed in the title bar of the chromatogram w

4 Operation 761 Compact IC 82 4.3.4 System settings Modify system window SYSTEM / Setup / Drag icons If this option is enabled, the system icons

4.3 Systems 761 Compact IC 83 Print system parameters SYSTEM / Setup / Parameters / Print A report of the system parameters including the time pr

4 Operation 761 Compact IC 84 Connected data source Data source for main measurement channel. By clicking on <choose>, the Data source wi

4.3 Systems 761 Compact IC 85 4.3.7 Instrument icon Menu options for instrument icon System disconnected System connectedInjection valve in &

4 Operation 761 Compact IC 86 Flow, mL/min Startup value for flow rate of the high-pressure pump. Entry range: 0.20 ... 2.50 mL/min Pressure ma

4.3 Systems 761 Compact IC 87 mouse button and selecting the menu item Choose color / ... . Actual values Display of actual values Flow, mL/min

4 Operation 761 Compact IC 88 Time program On the Program tab of the system settings window a user-defined time program for instrument control c

4.3 Systems 761 Compact IC 89 List of program instructions The following program instructions can be added to the time program on the Program subp